ARG63601
anti-KLF1 / EKLF antibody
anti-KLF1 / EKLF antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes KLF1 / EKLF |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Cow, Dog, Pig |
| 应用 | IHC-P |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KLF1 / EKLF |
| 抗原物种 | Human |
| 抗原 | ATAETALPSISTLT-C |
| 偶联标记 | Un-conjugated |
| 別名 | Krueppel-like factor 1; EKLF; CDAN4; INLU; Erythroid krueppel-like transcription factor; HBFQTL6 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 38 kDa |
| 翻译后修饰 | Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity (By similarity). Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex (By similarity). Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity (By similarity). |
检测图片 (1) Click the Picture to Zoom In
-
ARG63601 anti-KLF1 / EKLF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human bone marrow tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG63601 anti-KLF1 / EKLF antibody at 4 µg/ml dilution followed by HRP-staining.
