ARG41205
anti-KIAA0101 antibody
anti-KIAA0101 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes KIAA0101 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KIAA0101 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-111 of Human KIAA0101 (NP_055551.1). |
| 偶联标记 | Un-conjugated |
| 別名 | NS5ATP9; p15PAF; OEATC1; p15/PAF; PCNA-associated factor of 15 kDa; PCNA-associated factor; L5; p15(PAF); HCV NS5A-transactivated protein 9; PAF; OEATC-1; Overexpressed in anaplastic thyroid carcinoma 1; PAF15; OEATC; Hepatitis C virus NS5A-transactivated protein 9 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HT-29 | ||||
| 实际分子量 | 12 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KIAA0101 |
| 全名 | KIAA0101 |
| 生物功能 | PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm, perinuclear region. Note=Following DNA damage, localizes to DNA damage sites (PubMed:21628590). Colocalizes with centrosomes in perinuclear region (PubMed:21673012). [UniProt] |
| 预测分子量 | 12 kDa |
| 翻译后修饰 | Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG41205 anti-KIAA0101 antibody WB image
Western blot: 25 µg of HT-29 cell lysate stained with ARG41205 anti-KIAA0101 antibody at 1:1000 dilution.
