ARG53984
anti-KDM1 / LSD1 antibody
anti-KDM1 / LSD1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Monkey
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes KDM1A |
|---|---|
| 反应物种 | Hu, Mk |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | KDM1 / LSD1 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Flavin-containing amine oxidase domain-containing protein 2; Lysine-specific histone demethylase 1A; EC 1.-.-.-; BRAF35-HDAC complex protein BHC110; AOF2; KDM1; LSD1; BHC110 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 110 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O60341 Human Lysine-specific histone demethylase 1A |
|---|---|
| 基因名称 | KDM1A |
| 全名 | lysine (K)-specific demethylase 1A |
| 背景介绍 | Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3,thereby acting as a coactivator or a corepressor,depending on the context.Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.Acts as a corepressor by mediating demethylation of H3K4me,a specific tag for epigenetic transcriptional activation.Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me.May play a role in the repression of neuronal genes.Alone,it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription,by being recruited to ANDR target genes and mediating demethylation of H3K9me,a specific tag for epigenetic transcriptional repression.The presence of PRKCB in ANDR-containing complexes,which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph),a specific tag that prevents demethylation H3K4me,prevents H3K4me demethylase activity of KDM1A.Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation.Demethylates and stabilizes the DNA methylase DNMT1.Required for gastrulation during embryogenesis.Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses,via histone deacetylase (HDAC) recruitment,a number of genes implicated in multilineage blood cell development.Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1,CDN7 and KRT8.Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. |
| 生物功能 | Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 93 kDa |
| 翻译后修饰 | Polyubiquitinated by JADE2; which leads to its proteasomal degradation. |
检测图片 (3) Click the Picture to Zoom In
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ARG53984 anti-KDM1 / LSD1 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG53984 anti-KDM1 / LSD1 antibody at 1:100 dilution.
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ARG53984 anti-KDM1 / LSD1 antibody WB image
Western blot: HCT116 cell lysate stained with ARG53984 anti-KDM1 / LSD1 antibody at 1:1000 dilution.
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ARG53984 anti-KDM1 / LSD1 antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG53984 anti-KDM1 / LSD1 antibody.
