ARG23904
anti-KCNJ8 / kir6.1 antibody [S366-60]
anti-KCNJ8 / kir6.1 antibody [S366-60] for ICC/IF,Western blot and Human,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [S366-60] recognizes KCNJ8 / kir6.1 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S366-60 |
| 同位型 | IgG2a |
| 靶点名称 | KCNJ8 / kir6.1 |
| 抗原物种 | Rat |
| 抗原 | Fusion protein corresponding to aa. 306-424 (Cytoplasmic C-terminus) of Rat KCNJ8 / kir6.1. |
| 偶联标记 | Un-conjugated |
| 別名 | ATP-sensitive inward rectifier potassium channel 8; Potassium channel, inwardly rectifying subfamily J member 8; uKATP-1; KIR6.1; Inward rectifier K |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 40 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.1% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q15842 Human ATP-sensitive inward rectifier potassium channel 8 Swiss-port # Q63664 Rat ATP-sensitive inward rectifier potassium channel 8 |
|---|---|
| 基因名称 | KCNJ8 |
| 全名 | potassium channel, inwardly rectifying subfamily J, member 8 |
| 背景介绍 | Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. Defects in this gene may be a cause of J-wave syndromes and sudden infant death syndrome (SIDS). [provided by RefSeq, May 2012] |
| 生物功能 | This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium (By similarity). [UniProt] |
| 细胞定位 | Membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 48 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG23904 anti-KCNJ8 / kir6.1 antibody [S366-60] ICC/IF image
Immunofluorescence: Human neuroblastoma cell line SK-N-BE fixed with 4% Formaldehyde for 15 min at RT. Cells were stained with ARG23904 anti-KCNJ8 / kir6.1 antibody [S366-60] (green) at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Lower right picture: Composite.
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ARG23904 anti-KCNJ8 / kir6.1 antibody [S366-60] WB image
Western blot: 20 µg of Rat brain lysate stained with ARG23904 anti-KCNJ8 / kir6.1 antibody [S366-60] at 1:1000 for 16 hours at 4°C.
