ARG52400
anti-KCC2 / Potassium Chloride Cotransporter phospho (Ser940) antibody
anti-KCC2 / Potassium Chloride Cotransporter phospho (Ser940) antibody for Western blot and Rat
Neuroscience antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes KCC2 / Potassium Chloride Cotransporter phospho (Ser940) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Dog, NHuPrm |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | KCC2 / Potassium Chloride Cotransporter |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | K-Cl cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Solute carrier family 12 member 5; hKCC2; KCC2; Neuronal K-Cl cotransporter |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | Specific for the ~135k KCC2 protein phosphorylated at Ser940. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KCC2 |
| 全名 | solute carrier family 12 (potassium-chloride transporter), member 5 |
| 背景介绍 | KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it’s rate of internalization from the plasma membrane (Lee et al., 2007). |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 126 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG52400 anti-KCC2 / Potassium Chloride Cotransporter phospho (Ser940) antibody WB image
Western blot: Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control) stained with ARG52400 anti-KCC2 / Potassium Chloride Cotransporter phospho (Ser940) antibody.
Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide).
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