ARG65852
anti-JunB antibody
anti-JunB antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes JunB |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Recognizes endogenous levels of JunB protein. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | JunB |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide around the C-terminus of Human JunB. |
| 偶联标记 | Un-conjugated |
| 別名 | AP-1; Transcription factor jun-B |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Sodium citrate buffer (10mM, pH 6.0), Boiling bathing for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | Liquid (pH 7.3), 0.42% Potassium phosphate, 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | JUNB |
| 全名 | jun B proto-oncogene |
| 生物功能 | Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. [UniProt] |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | Ubiquitinated by ITCH, leading to its degradation. |
检测图片 (4) Click the Picture to Zoom In
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ARG65852 anti-JunB antibody WB image
Western blot: Rat lung stained with ARG65852 anti-JunB antibody and ARG51013 anti-NFkB p65 antibody.
From Jin F et al. J Inflamm Res- (2021), doi: 10.2147/JIR.S306883, Fig. 2. C.
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ARG65852 anti-JunB antibody ICC/IF image
Immunofluorescence: Formalin-fixed HEK293 cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 min and blocked with 3% BSA-PBS for 30 min at RT. Cells were stained with ARG65852 anti-JunB antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at RT in the dark. DAPI was used to stain the cell nuclei (blue).
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ARG65852 anti-JunB antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue section. Antigen retrieval: Sodium citrate buffer (10mM, pH 6.0), boiling bathing for 20 min. The section was stained with ARG65852 anti-JunB antibody at RT and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG65852 anti-JunB antibody WB image
Western blot: MCF7 whole cell lysates stained with ARG65852 anti-JunB antibody.
客户反馈
Average
anti-JunB antibody
Application：WB
Sample：MCF7
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
文献引用
Ac-SDKP Attenuates Activation of Lung Macrophages and Bone Osteoclasts in Rats Exposed to Silica by Inhibition of TLR4 and RANKL Signaling Pathways.
WB / Rat
