ARG53985
anti-JMJD6 antibody
anti-JMJD6 antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes JMJD6 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | JMJD6 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human JMJD6 (N-terminus) fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Peptide-lysine 5-dioxygenase JMJD6; JmjC domain-containing protein 6; Protein PTDSR; Phosphatidylserine receptor; PTDSR1; Lysyl-hydroxylase JMJD6; PTDSR; EC 1.14.11.-; PSR; Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; Jumonji domain-containing protein 6; Histone arginine demethylase JMJD6 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 62 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q6NYC1 Human Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 |
|---|---|
| 基因名称 | JMJD6 |
| 全名 | jumonji domain containing 6 |
| 背景介绍 | Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. |
| 生物功能 | Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 46 kDa |
检测图片 (2) Click the Picture to Zoom In
-
ARG53985 anti-JMJD6 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG53985 anti-JMJD6 antibody at 1:200 dilution.
-
ARG53985 anti-JMJD6 antibody WB image
Western blot: HL-60 cell lysate stained with ARG53985 anti-JMJD6 antibody at 1:1000 dilution.
