ARG62524
anti-Involucrin antibody [SY5]
anti-Involucrin antibody [SY5] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Dog,Gorilla,Monkey,Owl,Pig
Cancer antibody; Signaling Transduction antibody
参考文献1
概述
| 产品描述 | Mouse Monoclonal antibody [SY5] recognizes Involucrin |
|---|---|
| 反应物种 | Hu, Dog, Glla, Mk, Owl, Pig |
| 不反应物种 | Ms |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | This antibody stains involucrin in different sizes: 120 kDa in human keratinocytes, 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog (weak band) and a doublet of 105kDa in pig. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SY5 |
| 同位型 | IgG1 |
| 靶点名称 | Involucrin |
| 抗原物种 | Human |
| 抗原 | Pure involucrin from human keratinocytes |
| 抗原表位 | between codon 421-568 of human involucrin |
| 偶联标记 | Un-conjugated |
| 別名 | Involucrin |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified |
| 缓冲液 | 1X PBS buffer with < 0.1% sodium azide. |
| 抗菌剂 | < 0.1% sodium azide. |
| 浓度 | 2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IVL |
| 全名 | involucrin |
| 背景介绍 | Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008] |
| 生物功能 | Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. [UniProt] |
| 细胞定位 | Cytoplasm |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 68 kDa |
| 翻译后修饰 | Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. |
检测图片 (1) Click the Picture to Zoom In
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ARG62524 anti-Involucrin antibody [SY5] WB image
Western blot: HaCaT stained with ARG62524 anti-Involucrin antibody [SY5] at 1:1000 dilution.
From Chun-Yin Yang et al. Antioxidants (Basel). (2022), doi: 10.3390/antiox11112304, Fig. 7.
文献引用
Antioxidant, Anti-Inflammation and Antiaging Activities of Artocarpus altilis Methanolic Extract on Urban Particulate Matter-Induced HaCaT Keratinocytes Damage
WB / Human
