ARG44509

anti-Intersectin 1 antibody

anti-Intersectin 1 antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes Intersectin 1
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 Intersectin 1
抗原物种 Human
抗原 Human Intersectin 1 recombinant protein
偶联标记 Un-conjugated
別名 ITSN1; Intersectin 1; SH3P17; SH3D1A; ITSN; Human Intersectin-SH3 Domain-Containing Protein SH3P17; Src Homology 3 Domain-Containing Protein; Intersectin 1 (SH3 Domain Protein); SH3 Domain-Containing Protein 1A; Intersectin-1

应用说明

应用建议
应用 推荐稀释比
FACS1-3 µg/10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
应用说明 The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified with Immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6453 Human ITSN1

Swiss-port # Q15811 Human Intersectin-1

基因名称 ITSN1
全名 Intersectin 1
背景介绍 The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.
生物功能 Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery.
细胞定位 Cell membrane, Cell projection, Coated pit, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane, Nucleus, Synapse, Synaptosome
预测分子量 195 kDa
翻译后修饰 Phosphoprotein

检测图片 (3) Click the Picture to Zoom In

  • ARG44509 anti-Intersectin 1 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG44509 anti-Intersectin 1 antibody at 5 μg/mL dilution.

  • ARG44509 anti-Intersectin 1 antibody WB image

    Western blot: Caco-2, SiHa and HeLa stained with ARG44509 anti-Intersectin 1 antibody at 0.5 μg/mL dilution.

  • ARG44509 anti-Intersectin 1 antibody FACS image

    Flow Cytometry: SiHa stained with ARG44509 anti-Intersectin 1 antibody at 1 μg/10^6 cells dilution.