ARG64851
anti-ITM2B antibody
anti-ITM2B antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes ITM2B |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ITM2B |
| 抗原物种 | Human |
| 抗原 | C-HDKETYKLQRRETIK |
| 偶联标记 | Un-conjugated |
| 別名 | E25B; Bri2-23; Immature BRI2; BRICD2B; Bri; Integral membrane protein 2B; Protein E25B; BRI2; Mature BRI2; imBRI2; mBRI2; Transmembrane protein BRI; RDGCA; C-terminal peptide; BRI; FBD; BRI2 ICD; ABri23; P23 peptide; E3-16; ABRI |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia. [provided by RefSeq, Oct 2009] |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 30 kDa |
| 翻译后修饰 | The ectodomain C-terminal part of the imBRI2 is processed by furin producing a secreted Bri23 peptide and a mature BRI2, membrane form (mBRI2). The remaining part of the ectodomain of mBRI2 containing the BRICHOS domain is cleaved by ADAM10 and is secreted (BRI2C, soluble form). The membrane-bound N-terminal fragment (BRI2C, membrane form) is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted C-peptide and a BRI2 intracellular domain (BRI2 ICD) released in the cytosol. Shedding by ADAM10 facilitates intramembrane cleavage but is not absolutely required for BRI2 ICD generation. Glycosylation at Asn-170 is important for cell surface localization, but doesn't affect furin- and ADAM10-induced proteolytic processing. |
检测图片 (6) Click the Picture to Zoom In
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ARG64851 anti-ITM2B antibody WB image
Western Blot: Human Hippocampus lysate (35 µg protein in RIPA buffer) stained with ARG64851 anti-ITM2B antibody at 1 µg/ml dilution.
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ARG64851 anti-ITM2B antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A431 cells permeabilized with 0.15% Triton. Cells were stained with ARG64851 anti-ITM2B antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64851 anti-ITM2B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64851 anti-ITM2B antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG64851 anti-ITM2B antibody WB image
Western blot: 35 µg of HepG2 cell lysate (in RIPA buffer) stained with ARG64851 anti-ITM2B antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG64851 anti-ITM2B antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG64851 anti-ITM2B antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64851 anti-ITM2B antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64851 anti-ITM2B antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
