ARG63227
anti-IRF5 antibody
anti-IRF5 antibody for ChIP,Immunohistochemistry,Western blot and Human
Gene Regulation antibody; Immune System antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes IRF5 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow |
| 应用 | ChIP, IHC, WB |
| 特异性 | This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein: |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IRF5 |
| 抗原物种 | Human |
| 抗原 | C-QGPWPMHPAGMQ |
| 偶联标记 | Un-conjugated |
| 別名 | SLEB10; Interferon regulatory factor 5; IRF-5 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010] |
| 研究领域 | Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 56 kDa |
| 翻译后修饰 | Phosphorylation of serine and threonine residues in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/p300 and initiation of transcription. 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation. |
检测图片 (3) Click the Picture to Zoom In
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ARG63227 anti-IRF5 antibody WB image
Western Blot: A549 cell lysate (35 µg protein in RIPA buffer). stained with ARG63227 anti-IRF5 antibody at 0.1 µg/ml dilution.
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ARG63227 anti-IRF5 antibody WB image
Western Blot: 1). human IRF5 expressing plasmid transfected; 2) Mock transfection HEK293 cell lysate standed with ARG63227 anti-IRF5 antibody at 1ug/ml
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ARG63227 anti-IRF5 antibody WB image
Western blot: 30 µg of Human spleen (A) and peripheral blood lymphocytes (B) lysates (in RIPA buffer) stained with ARG63227 anti-IRF5 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
