ARG66755

anti-IRF4 / MUM1 antibody [SQab20188]

anti-IRF4 / MUM1 antibody [SQab20188] for IHC-Formalin-fixed paraffin-embedded sections and Human

概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab20188] recognizes IRF4 / MUM1
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab20188
同位型 IgG
靶点名称 IRF4 / MUM1
抗原物种 Human
抗原 Synthetic peptide around the C-terminus of Human IRF4 / MUM1.
偶联标记 Un-conjugated
別名 LSIRF; MUM1; Multiple myeloma oncogene 1; SHEP8; Interferon regulatory factor 4; NF-EM5; Lymphocyte-specific interferon regulatory factor; IRF-4

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Diffuse large B cell lymphoma tissue.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3662 Human IRF4

Swiss-port # Q15306 Human Interferon regulatory factor 4

基因名称 IRF4
全名 interferon regulatory factor 4
背景介绍 The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010]
生物功能 Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity). [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 52 kDa
翻译后修饰 Phosphorylation by ROCK2 regulates IL-17 and IL-21 production. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG66755 anti-IRF4 / MUM1 antibody [SQab20188] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human diffuse large B cell lymphoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66755 anti-IRF4 / MUM1 antibody [SQab20188] at 18°C - 25°C for 30 minutes.