ARG66967
anti-IRE1a antibody
anti-IRE1a antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IRE1a |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IRE1a |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the middle region of Human IRE1a protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Ire1-alpha; Serine/threonine-protein kinase/endoribonuclease IRE1; IRE1a; Endoplasmic reticulum-to-nucleus signaling 1; EC 3.1.26.-; IRE1; Inositol-requiring protein 1; IRE1P; EC 2.7.11.1; hIRE1p |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| 抗菌剂 | 0.025% ProClin 300 |
| 稳定剂 | 20% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O75460 Human Serine/threonine-protein kinase/endoribonuclease IRE1 Swiss-port # Q9EQY0 Mouse Serine/threonine-protein kinase/endoribonuclease IRE1 |
|---|---|
| 基因名称 | ERN1 |
| 全名 | endoplasmic reticulum to nucleus signaling 1 |
| 背景介绍 | IRE1p Antibody: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| 生物功能 | Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 110 kDa |
| 翻译后修饰 | Autophosphorylated. ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities. |
检测图片 (3) Click the Picture to Zoom In
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ARG66967 anti-IRE1a antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human Tonsil cancer tissue stained with ARG66967 anti-IRE1a antibody at 1:100 dilution.
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ARG66967 anti-IRE1a antibody WB image
Western blot: Lysate from HepG2 treated with thapsigargin (TG) or not, stained with ARG66967 anti-IRE1a antibody at 1:500 dilution, overnight at 4°C.
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ARG66967 anti-IRE1a antibody WB image
Western blot: C2C12 cell lysate stained with ARG66967 anti-IRE1a antibody at 1:500 dilution, overnight at 4°C.
