ARG63779
anti-IRAK4 antibody
anti-IRAK4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes IRAK4 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IRAK4 |
| 抗原物种 | Human |
| 抗原 | NKPITPSTYVRC |
| 偶联标记 | Un-conjugated |
| 別名 | REN64; Renal carcinoma antigen NY-REN-64; NY-REN-64; Interleukin-1 receptor-associated kinase 4; EC 2.7.11.1; IRAK-4; IPD1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NWZ3 Human Interleukin-1 receptor-associated kinase 4 |
|---|---|
| 背景介绍 | This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Phosphorylated. |
检测图片 (6) Click the Picture to Zoom In
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ARG63779 anti-IRAK4 antibody WB image
Western Blot: HeLa lysate (35 µg protein in RIPA buffer) stained with ARG63779 anti-IRAK4 antibody at 1 µg/ml dilution.
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ARG63779 anti-IRAK4 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63779 anti-IRAK4 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63779 anti-IRAK4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63779 anti-IRAK4 antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG63779 anti-IRAK4 antibody WB image
Western blot: 35 µg of HeLa (A) and Jurkat (B) cell lysates (in RIPA buffer) stained with ARG63779 anti-IRAK4 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63779 anti-IRAK4 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63779 anti-IRAK4 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG63779 anti-IRAK4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63779 anti-IRAK4 antibody at 3.75 µg/ml dilution followed by AP-staining.
