ARG54631
anti-IRAK4 antibody
anti-IRAK4 antibody for ICC/IF,Western blot and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IRAK4 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 特异性 | This antibody specifically recognizeshuman IRAK-4 (IL-1 ReceptorAssociated Kinase-4). This antibodydoes not cross-react with other IRAKs. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IRAK4 |
| 抗原物种 | Human |
| 抗原 | A synthetic peptidecorresponding to amino acids at thecarboxy terminus of human IRAK-4. |
| 偶联标记 | Un-conjugated |
| 別名 | REN64; Renal carcinoma antigen NY-REN-64; NY-REN-64; Interleukin-1 receptor-associated kinase 4; EC 2.7.11.1; IRAK-4; IPD1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa and K562 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | purified by Immunoaffinity chromatography. |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NWZ3 Human Interleukin-1 receptor-associated kinase 4 |
|---|---|
| 基因名称 | IRAK4 |
| 全名 | interleukin-1 receptor-associated kinase 4 |
| 背景介绍 | IRAK-4 activates the NF-B and MAPKpathways and plays a role in IL-1Rmediated inflammatory responses andinnate immunity. IRAK-4-deficientanimals are resistant to challenge withLPS and are impaired in their responsesto viral and bacterial challenges. |
| 生物功能 | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. [UniProt] |
| 产品亮点 | Related products: IRAK4 antibodies; Anti-Rabbit IgG secondary antibodies; Related poster download: The NF-kappa B Pathways.pdf Toll-like Receptor.pdf |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 52 kDa |
| 翻译后修饰 | Phosphorylated. |
检测图片 (3) Click the Picture to Zoom In
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ARG54631 anti-IRAK4 antibody WB image
Western Blot: IRAK-4 in HeLa cell lysate stained with ARG54631 anti-IRAK4 antibody at 1, 2, and 3μg/ml dilution.
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ARG54631 anti-IRAK4 antibody ICC/IF image
Immunofluorescence: K562 stained with ARG54631 anti-IRAK4 antibody at 10 μg/ml dilution.
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ARG54631 anti-IRAK4 antibody ICC/IF image
Immunofluorescence: K562 stained with ARG54631 anti-IRAK4 antibody at 10 μg/ml dilution.
客户反馈
Average
anti-IRAK4 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
