ARG45758
anti-INSIG1 antibody
anti-INSIG1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes INSIG1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | INSIG1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human INSIG1. |
| 偶联标记 | Un-conjugated |
| 別名 | INSIG1; Insulin Induced Gene 1; Insulin-Induced Gene 1 Protein; INSIG-1 Membrane Protein; MGC1405; CL-6; INSIG-1; CL6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 66 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | INSIG1 |
| 全名 | Insulin Induced Gene 1 |
| 背景介绍 | This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| 生物功能 | Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR. [UniProt] |
| 细胞定位 | Endoplasmic reticulum; Membrane. [UniProt] |
| 预测分子量 | 30 kDa |
| 翻译后修饰 | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG45758 anti-INSIG1 antibody IHC-P image
Immunohistochemistry: Human liver cancer stained with ARG45758 anti-INSIG1 antibody at 2 μg/ml dilution.
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ARG45758 anti-INSIG1 antibody ICC/IF image
Immunofluorescence: Hela stained with ARG45758 anti-INSIG1 antibody at 5 μg/ml dilution.
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ARG45758 anti-INSIG1 antibody WB image
Western blot: HepG2 stained with ARG45758 anti-INSIG1 antibody at 0.5 μg/ml dilution.
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ARG45758 anti-INSIG1 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45758 anti-INSIG1 antibody at 1 µg/10^6 cells dilution.
