ARG59926
anti-ING4 antibody
anti-ING4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ING4 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 特异性 | The antibody might also react to ING5 based on sequence homology analysis (64%). |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ING4 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-190 of Human ING4 (NP_001121054.1). |
| 偶联标记 | Un-conjugated |
| 別名 | p29ING4; my036; Inhibitor of growth protein 4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse spleen and A549 | ||||||
| 实际分子量 | 52 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ING4 |
| 全名 | inhibitor of growth family, member 4 |
| 背景介绍 | This gene encodes a tumor suppressor protein that contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed that encode distinct proteins. [provided by RefSeq, Jul 2008] |
| 生物功能 | Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). Can enhance aopotosis induced by serum starvation in mammary epithelial cell line HC11 (By similarity). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Citrullination by PADI4 within the nuclear localization signal disrupts the interaction with p53 and increases susceptibility to degradation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59926 anti-ING4 antibody WB image
Western blot: 25 µg of Mouse spleen and A549 cell lysates stained with ARG59926 anti-ING4 antibody at 1:1000 dilution.
