ARG40399
anti-ILK antibody
anti-ILK antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ILK |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ILK |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human ILK. |
| 偶联标记 | Un-conjugated |
| 別名 | ILK-1; ILK-2; p59ILK; 59 kDa serine/threonine-protein kinase; P59; Integrin-linked protein kinase; EC 2.7.11.1; HEL-S-28 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ILK |
| 全名 | integrin-linked kinase |
| 背景介绍 | This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
| 生物功能 | Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. [UniProt] |
| 细胞定位 | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cytoplasm, myofibril, sarcomere. [UniProt] |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Autophosphorylated on serine residues. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40399 anti-ILK antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG40399 anti-ILK antibody.
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ARG40399 anti-ILK antibody WB image
Western blot: K562 cell lysate stained with ARG40399 anti-ILK antibody.
