ARG10006

anti-IL8 antibody [I8-60]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

产品说明书

概述

产品描述	Mouse Monoclonal antibody [I8-60] recognizes IL8
反应物种	Hu
应用	ELISA, IHC-P, Neut, WB
特异性	Does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
宿主	Mouse
克隆	Monoclonal
克隆号	I8-60
同位型	IgG1, kappa
靶点名称	IL8
抗原物种	Human
抗原	Purified recombinant human IL-8
偶联标记	Un-conjugated
別名	IL8/NAP1 form IV; GCP/IL-8 protein IV; NAF; T-cell chemotactic factor; 1-77; Ala-IL-8; Interleukin-8; IL-8; Neutrophil-activating protein 1; GCP/IL-8 protein II; IL8/NAP1 form II; GCP/IL-8 protein V; MDNCF; Protein 3-10C; Lymphocyte-derived neutrophil-activating factor; Neutrophil-activating factor; Granulocyte chemotactic protein 1; LYNAP; NAP-1; Monocyte-derived neutrophil chemotactic factor; 6-77; 7-77; C-X-C motif chemokine 8; GCP1; NAP1; Ser-IL-8; 5-77; GCP/IL-8 protein VI; IL8/NAP1 form I; IL8/NAP1 form VI; Monocyte-derived neutrophil-activating peptide; C-X-C motif; 8-77; 9-77; LUCT; Chemokine; GCP-1; MDNCF-b; MDNCF-c; IL8/NAP1 form V; LECT; IL8/NAP1 form III; GCP/IL-8 protein III; Emoctakin; GCP/IL-8 protein I; MONAP; IL8

应用说明

应用说明	ELISA: This antibody can be used as a tracer antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: ARG10005). Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5μg/mL of antibody and 10 ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes. Western Blot: Concentration of 0.02-0.1μg/mL antibody will allow visualization of 100 ng/lane of human IL-8. IHC-P: Used as an assay-dependent dilution. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

ELISA: This antibody can be used as a tracer antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: ARG10005).

Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5μg/mL of antibody and 10 ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.

Western Blot: Concentration of 0.02-0.1μg/mL antibody will allow visualization of 100 ng/lane of human IL-8.

IHC-P: Used as an assay-dependent dilution.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Protein G affinity purified
缓冲液	0.01M PBS (pH 7.0)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3576 Human CXCL8 Swiss-port # P10145 Human Interleukin-8
基因名称	CXCL8
全名	chemokine (C-X-C motif) ligand 8
背景介绍	Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.
生物功能	IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. [UniProt]
产品亮点	Related products: IL8 antibodies; IL8 ELISA Kits; IL8 Duos / Panels; IL8 recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody
预测分子量	11 kDa
翻译后修饰	Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.

检测图片 (2) Click the Picture to Zoom In

ARG10006 anti-IL8 antibody [I8-60] WB image

Western Blot: Recombinant human IL-8 stained with anti-IL8 antibody [I8-60] (ARG10006)
ARG10006 anti-IL8 antibody [I8-60] IHC-P image

Immunohistochemistry: Stratum corneum of psoriatic skin tissue stained with ARG10006 anti-IL8 antibody [I8-60] followed by incubation with Biotinylated secondary antibody and avidin-HRP. The specimen was stained with DAB substrate for 5~10 min, and counter-stained with hematoxylin.