ARG66157
anti-IL36 gamma antibody (Biotin)
anti-IL36 gamma antibody (Biotin) for ELISA,Western blot and Human
概述
| 产品描述 | Biotin-conjugated Rabbit Polyclonal antibody recognizes IL36 gamma |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IL36 gamma |
| 抗原物种 | Human |
| 抗原 | E. coli derived recombinant Human IL36 gamma. (SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND) |
| 偶联标记 | Biotin |
| 別名 | IL-1 epsilon; IL1H1; Interleukin-1 homolog 1; IL-1-related protein 2; Interleukin-36 gamma; IL-1H1; IL-1F9; IL1F9; IL-1RP2; IL1RP2; IL1E; Interleukin-1 family member 9; Interleukin-1 epsilon |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by affinity chromatography. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | IL36G |
| 全名 | interleukin 36, gamma |
| 背景介绍 | The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] |
| 生物功能 | Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. [UniProt] |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). |
检测图片 (3) Click the Picture to Zoom In
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ARG66157 anti-IL36 gamma antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human IL-36γ stained with ARG66157 anti-IL36 gamma antibody (Biotin), under non-reducing conditions.
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ARG66157 anti-IL36 gamma antibody (Biotin) standard curve image
Sandwich ELISA: ARG66157 anti-IL36 gamma antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66156 anti-IL36 gamma antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66157 anti-IL36 gamma antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human IL-36γ stained with ARG66157 anti-IL36 gamma antibody (Biotin), under reducing conditions.
