ARG66425
anti-IL33 antibody [SQab18127]
anti-IL33 antibody [SQab18127] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [SQab18127] recognizes IL33 |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SQab18127 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | IL33 |
| 抗原物种 | Human |
| 抗原 | Recombinant Human IL33 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | 95-270; NF-HEV; Interleukin-33; C9orf26; IL1F11; 99-270; Interleukin-1 family member 11; IL-33; IL-1F11; Nuclear factor from high endothelial venules; NFEHEV; DVS27; 109-270 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 纯化 | Affinity purification with immunogen. |
|---|---|
| 缓冲液 | PBS (pH 7.4) and 0.01% Thimerosal. |
| 抗菌剂 | 0.01% Thimerosal |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL33 |
| 全名 | interleukin 33 |
| 背景介绍 | The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. [UniProt] |
| 细胞定位 | Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Note=Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). [UniProt] |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG66425 anti-IL33 antibody [SQab18127] ICC/IF image
Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66425 anti-IL33 antibody [SQab18127] (green) at 1:400 dilution and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66425 anti-IL33 antibody [SQab18127] FACS image
Flow Cytometry: HeLa cells were stained with ARG66425 anti-IL33 antibody [SQab18127] at 1 µg/ml (red) and without antibody control (black).
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ARG66425 anti-IL33 antibody [SQab18127] WB image
Western blot: 50 ng of Recombinant IL33 protein stained with ARG66425 anti-IL33 antibody [SQab18127] at 1:5000 dilution.
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ARG66425 anti-IL33 antibody [SQab18127] ELISA image
ELISA: Titration curve of ARG66425 anti-IL33 antibody [SQab18127]. Red: IL33; Black: BSA (negative control).
