ARG56952
anti-IL33 antibody [4E9]
anti-IL33 antibody [4E9] for Flow cytometry,ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [4E9] recognizes IL33 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4E9 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | IL33 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 112-270 of Human IL33. |
| 偶联标记 | Un-conjugated |
| 別名 | 95-270; NF-HEV; Interleukin-33; C9orf26; IL1F11; 99-270; Interleukin-1 family member 11; IL-33; IL-1F11; Nuclear factor from high endothelial venules; NFEHEV; DVS27; 109-270 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL33 |
| 全名 | interleukin 33 |
| 背景介绍 | The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. [UniProt] |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. |
检测图片 (4) Click the Picture to Zoom In
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ARG56952 anti-IL33 antibody [4E9] ICC/IF image
Immunoflorescense: Jurkat cell line stained with ARG56952 anti-IL33 antibody [4E9] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56952 anti-IL33 antibody [4E9] FACS image
Flow Cytometry: Jurkat cell line stained with ARG56952 anti-IL33 antibody [4E9] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG56952 anti-IL33 antibody [4E9] WB image
Western blot: 40 µg of 293T cell lysate stained with ARG56952 anti-IL33 antibody [4E9] at 1:1000.
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ARG56952 anti-IL33 antibody [4E9] WB image
Western blot: 35 µg of Jurkat cell lysate stained with ARG56952 anti-IL33 antibody [4E9] at 1:500.
