ARG66015

anti-IL3 antibody

anti-IL3 antibody for ELISA,Neutralizing,Western blot and Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes IL3
反应物种 Ms
应用 ELISA, Neut, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 IL3
抗原物种 Mouse
抗原 E. coli derived recombinant Mouse IL3.
(MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC)
偶联标记 Un-conjugated
別名 MCGF; Mast cell growth factor; P-cell-stimulating factor; Hematopoietic growth factor; IL-3; Interleukin-3; Multipotential colony-stimulating factor; MULTI-CSF

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66016 as a detection antibody
Neut0.15 - 0.20 µg/ml (To yield [ND50] of the biological activity of mIL-3 (0.5 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 16187 Mouse IL3

Swiss-port # P01586 Mouse Interleukin-3

基因名称 Il3
全名 interleukin 3
背景介绍 The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
生物功能 Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. [UniProt]
预测分子量 17 kDa

检测图片 (5) Click the Picture to Zoom In

  • ARG66015 anti-IL3 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-3 stained with ARG66015 anti-IL3 antibody, under non-reducing conditions.

  • ARG66015 anti-IL3 antibody standard curve image

    Sandwich ELISA: ARG66015 anti-IL3 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66016 anti-IL3 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66015 anti-IL3 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-3 stained with ARG66015 anti-IL3 antibody, under reducing conditions.

  • ARG66015 anti-IL3 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-3 stained with ARG66015 anti-IL3 antibody, under reducing conditions.

  • ARG66015 anti-IL3 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-3 stained with ARG66015 anti-IL3 antibody, under non-reducing conditions.