ARG41569

anti-IL18 antibody

anti-IL18 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

概述

产品描述 Rabbit Polyclonal antibody recognizes IL18
反应物种 Ms
应用 IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 IL18
抗原物种 Mouse
抗原 Recombinant protein corresponding to N36-S192 of Mouse IL18.
偶联标记 Un-conjugated
別名 Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4

应用说明

应用建议
应用 推荐稀释比
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 22 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 0.05% Sodium azide.
抗菌剂 0.05% Sodium azide
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 16173 Mouse IL18

Swiss-port # P70380 Mouse Interleukin-18

基因名称 IL18
全名 interleukin 18
背景介绍 The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
生物功能 Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt]
细胞定位 Secreted. [UniProt]
预测分子量 22 kDa
翻译后修饰 The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG41569 anti-IL18 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41569 anti-IL18 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG41569 anti-IL18 antibody WB image

    Western blot: 10 ng, 5 ng, 2.5 ng and 1.25 ng of Recombinant Mouse IL18 Protein stained with ARG41569 anti-IL18 antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.

  • ARG41569 anti-IL18 antibody WB image

    Western blot: 50 μg of Mouse thymus lysate stained with ARG41569 anti-IL18 antibody at 0.5 μg/ml dilution, overnight at 4°C, under reducing condition.