ARG41565
anti-IL18 antibody
anti-IL18 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IL18 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IL18 |
| 抗原物种 | Rat |
| 抗原 | Recombinant protein corresponding to H37-S194 of Rat IL18. |
| 偶联标记 | Un-conjugated |
| 別名 | Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 22 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL18 |
| 全名 | interleukin 18 |
| 背景介绍 | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011] |
| 生物功能 | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 22 kDa |
| 翻译后修饰 | The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG41565 anti-IL18 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41565 anti-IL18 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG41565 anti-IL18 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain, Rat lung, Rat spleen, Rat liver, Rat testis, RH35 and PC-12 whole cell lysates stained with ARG41565 anti-IL18 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41565 anti-IL18 antibody WB image
Western blot: 30 µg of sample under reducing conditions. Mouse spleen, Mouse thymus, Mouse lung and ANA-1 whole cell lysates stained with ARG41565 anti-IL18 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41565 anti-IL18 antibody FACS image
Flow Cytometry: RH35 cells were blocked with 10% normal goat serum and then stained with ARG41565 anti-IL18 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
