ARG41564
anti-IL18 antibody
anti-IL18 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
产品描述 | Rabbit Polyclonal antibody recognizes IL18 |
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反应物种 | Hu |
应用 | ICC/IF, IHC-P, WB |
宿主 | Rabbit |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | IL18 |
抗原物种 | Human |
抗原 | Recombinant protein corresponding to Y37-D193 of Human IL18. |
偶联标记 | Un-conjugated |
別名 | Iboctadekin; Interferon gamma-inducing factor; Interleukin-18; IL-18; IFN-gamma-inducing factor; IL-1 gamma; IGIF; IL-1g; Interleukin-1 gamma; IL1F4 |
应用说明
应用建议 |
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应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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实际分子量 | ~ 22 kDa |
属性
形式 | Liquid |
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纯化 | Affinity purification with immunogen. |
缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
抗菌剂 | 0.05% Sodium azide |
稳定剂 | 5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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基因名称 | IL18 |
全名 | interleukin 18 |
背景介绍 | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011] |
生物功能 | Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. [UniProt] |
细胞定位 | Secreted. [UniProt] |
预测分子量 | 22 kDa |
翻译后修饰 | The pro-IL-18 precursor is processed by CASP1 or CASP4 to yield the active form. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG41564 anti-IL18 antibody ICC image
Immunocytochemistry: Blood cells stained with ARG41564 anti-IL18 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41564 anti-IL18 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41564 anti-IL18 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41564 anti-IL18 antibody WB image
Western blot: 50 µg of sample under reducing conditions. HeLa whole cell lysate stained with ARG41564 anti-IL18 antibody at 0.5 µg/ml, overnight at 4°C.