ARG66284

anti-IL16 antibody [SQab1747]

anti-IL16 antibody [SQab1747] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

概述

产品描述 Mouse Monoclonal antibody [SQab1747] recognizes IL16
反应物种 Hu, Ms
预测物种 Rat
应用 ELISA, FACS, ICC/IF, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 SQab1747
同位型 IgG1
靶点名称 IL16
抗原物种 Human
抗原 Recombinant Human IL16 protein.
偶联标记 Un-conjugated
別名 prIL-16; Lymphocyte chemoattractant factor; PRIL16; IL-16; NIL16; LCF; Pro-interleukin-16

应用说明

应用建议
应用 推荐稀释比
ELISA1:3000 - 1:10000
FACS1:400 - 1:1000
ICC/IF1:100 - 1:500
IHC-P1:3000 - 1:10000
WB1:2500 - 1:5000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂 0.01% Thimerosal
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 16170 Mouse IL16

GeneID: 3603 Human IL16

Swiss-port # O54824 Mouse Pro-interleukin-16

Swiss-port # Q14005 Human Pro-interleukin-16

基因名称 IL16
全名 interleukin 16
背景介绍 The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
生物功能 Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.

Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.

Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. [UniProt]
预测分子量 142 kDa
翻译后修饰 Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG66284 anti-IL16 antibody [SQab1747] ICC/IF image

    Immunofluorescence: A549 cells stained with ARG66284 anti-IL16 antibody [SQab1747] at 1:200 dilution.

  • ARG66284 anti-IL16 antibody [SQab1747] FACS image

    Flow Cytometry: HeLa cells stained with ARG66284 anti-IL16 antibody [SQab1747] at 1 µg/ml (red) and without antibody control (black).

  • ARG66284 anti-IL16 antibody [SQab1747] WB image

    Western blot: 20 µg of HeLa and 30 µg of K562 cell lysates stained with ARG66284 anti-IL16 antibody [SQab1747] at 1:5000 dilution.

  • ARG66284 anti-IL16 antibody [SQab1747] ELISA image

    ELISA: Titration curve of ARG66284 anti-IL16 antibody [SQab1747] in ELISA. Red: IL16; Black: BSA (negative control).

  • ARG66284 anti-IL16 antibody [SQab1747] FACS image

    Flow Cytometry: K562 cells stained with ARG66284 anti-IL16 antibody [SQab1747] at 1 µg/ml (red) and without antibody control (black).

  • ARG66284 anti-IL16 antibody [SQab1747] WB image

    Western blot: 50 µg of Mouse brain lysate stained with ARG66284 anti-IL16 antibody [SQab1747] at 1:5000 dilution.