ARG56745

anti-IL12 antibody (Biotin)

anti-IL12 antibody (Biotin) for ELISA,Western blot and Human

概述

产品描述 Biotin-conjugated Goat Polyclonal antibody recognizes IL12
反应物种 Hu
应用 ELISA, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 IL12
抗原物种 Human
抗原 CHO cells derived Recombinant Human IL-12.
(p35 Subunit: RNLPVA TPDPGMFPCL HHSQNLLRAV SNMLQKARQT LEFYPCTSEE IDHEDITKDK TSTVEACLPL ELTKNESCLN SRETSFITNG SCLASRKTSF MMALCLSSIY EDLKMYQVEF KTMNAKLLMD PKRQIFLDQN MLAVIDELMQ ALNFNSETVP QKSSLEEPDF YKTKIKLCIL LHAFRIRAVT IDRVMSYLNA S p40 Subunit: IWELKK DVYVVELDWY PDAPGEMVVL TCDTPEEDGI TWTLDQSSEV LGSGKTLTIQ VKEFGDAGQY TCHKGGEVLS HSLLLLHKKE DGIWSTDILK DQKEPKNKTF LRCEAKNYSG RFTCWWLTTI STDLTFSVKS SRGSSDPQGV TCGAATLSAE RVRGDNKEYE YSVECQEDSA CPAAEESLPI EVMVDAVHKL KYENYTSSFF IRDIIKPDPP KNLQLKPLKN SRQVEVSWEY PDTWSTPHSY FSLTFCVQVQ GKSKREKKDR VFTDKTSATV ICRKNASISV RAQDRYYSSS WSEWASVPCS)
偶联标记 Biotin
別名 CLMF; Cytotoxic lymphocyte maturation factor 35 kDa subunit; NFSK; NK cell stimulatory factor chain 1; CLMF p35; NKSF1; IL-12 subunit p35; IL-12A; Interleukin-12 subunit alpha; P35

应用说明

应用建议
应用 推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56635 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by affinity chromatography.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3592 Human IL12A

Swiss-port # P29459 Human Interleukin-12 subunit alpha

基因名称 IL12A
全名 interleukin 12A
背景介绍 This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]
生物功能 Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. [UniProt]
预测分子量 25 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG56745 anti-IL12 antibody (Biotin) standard curve image

    Direct ELISA: ARG56745 anti-IL12 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56745 anti-IL12 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human IL-12 stained with ARG56745 anti-IL12 antibody (Biotin), under non-reducing conditions.

  • ARG56745 anti-IL12 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56745 anti-IL12 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56635 anti-IL12 antibody as a capture antibody. Results of a typical standard run with optical density.