anti-IL1 beta antibody
anti-IL1 beta antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Immunoprecipitation and Human,Mouse,Rat
|产品描述||Rabbit Polyclonal antibody recognizes IL1 beta|
|反应物种||Hu, Ms, Rat|
|应用||ICC/IF, IHC-P, IP, WB|
|抗原||Synthetic peptide of Human IL1 beta.|
|別名||Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2|
|应用说明||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|实际分子量||17, 35 kDa|
|缓冲液||PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.|
|抗菌剂||0.02% Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||interleukin 1, beta|
|背景介绍||The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]|
|生物功能||Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]|
|细胞定位||Cytoplasm, cytosol. Lysosome. Secreted, exosome. Secreted. Note=The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood. [UniProt]|
|翻译后修饰||Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. [UniProt]|
检测图片 (4) Click the Picture to Zoom In
ARG43441 anti-IL1 beta antibody ICC/IF image
Immunofluorescence: THP-1 cells stained with ARG43441 anti-IL1 beta antibody at 1:100 dilution.
ARG43441 anti-IL1 beta antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat lung tissue stained with ARG43441 anti-IL1 beta antibody at 1:100 dilution.
ARG43441 anti-IL1 beta antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG43441 anti-IL1 beta antibody at 1:1000 dilution.
ARG43441 anti-IL1 beta antibody IP image
Immunoprecipitation: 300 µg extracts of THP-1 cells were immunoprecipitated and stained with ARG43441 anti-IL1 beta antibody at 1:1000 dilution.