ARG56778

anti-IL1 alpha antibody (Biotin)

anti-IL1 alpha antibody (Biotin) for ELISA,Western blot and Rat

概述

产品描述 Biotin-conjugated Goat Polyclonal antibody recognizes IL1 alpha
反应物种 Rat
应用 ELISA, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 IL1 alpha
抗原物种 Rat
抗原 E.coli derived Recombinant Rat IL-1α.
(MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS)
偶联标记 Biotin
別名 IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

应用说明

应用建议
应用 推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56668 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by affinity chromatography.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 24493 Rat IL1A

Swiss-port # P16598 Rat Interleukin-1 alpha

基因名称 Il1a
全名 interleukin 1 alpha
背景介绍 The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
生物功能 Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
产品亮点 Related products:
IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins; Anti-Goat IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
预测分子量 31 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG56778 anti-IL1 alpha antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Rat IL-1α stained with ARG56778 anti-IL1 alpha antibody (Biotin), under reducing conditions.

  • ARG56778 anti-IL1 alpha antibody (Biotin) standard curve image

    Direct ELISA: ARG56778 anti-IL1 alpha antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56778 anti-IL1 alpha antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Rat IL-1α stained with ARG56778 anti-IL1 alpha antibody (Biotin), under non-reducing conditions.

  • ARG56778 anti-IL1 alpha antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56778 anti-IL1 alpha antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56668 anti-IL1 alpha antibody as a capture antibody. Results of a typical standard run with optical density.