ARG51651

anti-IKB alpha phospho (Ser32 / Ser36) antibody

anti-IKB alpha phospho (Ser32 / Ser36) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody; NF-kB Activation Study antibody
publication_link 参考文献2

概述

产品描述 Rabbit Polyclonal antibody recognizes IKB alpha phospho (Ser32 / Ser36)
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 IKB alpha
抗原物种 Human
抗原 Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IκB-α.
偶联标记 Un-conjugated
別名 NFKBI; I-kappa-B-alpha; IkB-alpha; NF-kappa-B inhibitor alpha; Major histocompatibility complex enhancer-binding protein MAD3; IKBA; IkappaBalpha; MAD-3

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 18035 Mouse NFKBIA

GeneID: 25493 Rat NFKBIA

GeneID: 4792 Human NFKBIA

基因名称 NFKBIA
全名 nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
背景介绍 Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
生物功能 Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30037 Phospho IKB alpha Antibody Duo (Total, pS32/36)
ARG30205 NFkB Activation Antibody Panel
Related products:
IKB alpha antibodies; IKB alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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研究领域 Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody; NF-kB Activation Study antibody
预测分子量 36 kDa
翻译后修饰 Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

检测图片 (4) Click the Picture to Zoom In

  • ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image

    Western blot: Rat cardiac stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody at dilution.

    From Yongjun Mao et al. Biomed Pharmacother. (2020), doi: 10.1016/j.biopha.2019.109280, Fig. 6D.

  • ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image

    Western blot: Extracts from HeLa cells untreated or treated with TNF stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.

  • ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody ICC/IF image

    Immunofluorescence: methanol-fixed HeLa cells stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.

文献引用

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

WB / Human

Yuhua Zhou et al.
Cent Eur J Immunol .,  (2022)

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Effects and mechanisms of PSS-loaded nanoparticles on coronary microcirculation dysfunction in streptozotocin-induced diabetic cardiomyopathy rats.

WB / Rat

Mao Yongjun et al.
Biomed Pharmacother.,  (2019)

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