ARG40917
anti-IGFBP4 antibody
anti-IGFBP4 antibody for Flow cytometry,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IGFBP4 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Bov, Pig |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IGFBP4 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 66-92 of Human IGFBP4. |
| 偶联标记 | Un-conjugated |
| 別名 | IBP4; IBP-4; IGFBP-4; Insulin-like growth factor-binding protein 4; HT29-IGFBP; IGF-binding protein 4; BP-4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 28 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IGFBP4 |
| 全名 | insulin-like growth factor binding protein 4 |
| 背景介绍 | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008] |
| 生物功能 | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 28 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG40917 anti-IGFBP4 antibody WB image
Western blot: 20 µg of Mouse ovary lysate stained with ARG40917 anti-IGFBP4 antibody at 1:2000 dilution.
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ARG40917 anti-IGFBP4 antibody FACS image
Flow Cytometry: A549 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG40917 anti-IGFBP4 antibody (green) at 1:25 dilution for 60 min at 37°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (blue) was Rabbit IgG1 (1 ug/10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
