ARG56665

anti-IGF1 antibody

anti-IGF1 antibody for ELISA,Neutralizing,Western blot and Mouse

概述

产品描述 Goat Polyclonal antibody recognizes IGF1
反应物种 Ms
应用 ELISA, Neut, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 IGF1
抗原物种 Mouse
抗原 E.coli derived Recombinant Mouse IGF1.
(GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA)
偶联标记 Un-conjugated
別名 MGF; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C; IGFI; IGF-I

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56775 as a detection antibody
Neut12.0 - 14.0 µg/ml (To yield [ND50] of the biological activity of Murine IGF - I (200 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 16000 Mouse IGF1

Swiss-port # P05017 Mouse Insulin-like growth factor I

基因名称 Igf1
全名 insulin-like growth factor 1
背景介绍 The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]
生物功能 The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. [UniProt]
预测分子量 22 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG56665 anti-IGF1 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IGF-I stained with ARG56665 anti-IGF1 antibody, under reducing conditions.

  • ARG56665 anti-IGF1 antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IGF-I stained with ARG56665 anti-IGF1 antibody, under non-reducing conditions.

  • ARG56665 anti-IGF1 antibody standard curve image

    Sandwich ELISA: ARG56665 anti-IGF1 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56775 anti-IGF1 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.