ARG43398
anti-IFN gamma antibody (Biotin)
anti-IFN gamma antibody (Biotin) for ELISA,Western blot and Mouse
概述
| 产品描述 | Biotin-conjugated Rabbit Polyclonal antibody recognizes IFN gamma |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IFN gamma |
| 抗原物种 | Mouse |
| 抗原 | E. coli-derived recombinant Mouse IFN gamma. |
| 偶联标记 | Biotin |
| 別名 | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | Sandwich ELISA (Capture antibody - Detection antibody): ARG56459 - ARG43398 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IFNG |
|---|---|
| 全名 | interferon, gamma |
| 背景介绍 | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015] |
| 生物功能 | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG43398 anti-IFN gamma antibody (Biotin) WB image
Western blot: 3.0 - 1.5 ng (left to right) of Recombinant Murine IFN gamma protein stained with ARG43398 anti-IFN gamma antibody (Biotin), under reducing conditions.
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ARG43398 anti-IFN gamma antibody (Biotin) standard curve image
Sandwich ELISA: ARG43398 anti-IFN gamma antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56459 anti-IFN gamma antibody as a capture antibody. Results of a typical standard run with optical density reading at 450 - 620 nm.
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ARG43398 anti-IFN gamma antibody (Biotin) WB image
Western blot: 3.0 - 1.5 ng (left to right) of Recombinant Murine IFN gamma protein stained with ARG43398 anti-IFN gamma antibody (Biotin), under non-reducing conditions.
