ARG56608

anti-IFN gamma antibody

anti-IFN gamma antibody for ELISA,Neutralizing,Western blot and Rat

概述

产品描述 Goat Polyclonal antibody recognizes IFN gamma
反应物种 Rat
应用 ELISA, Neut, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 IFN gamma
抗原物种 Rat
抗原 E.coli derived Recombinant Rat IFN-γ.
(MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC)
偶联标记 Un-conjugated
別名 IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56723 as a detection antibody
Neut60.0 - 65.0 ng/ml (To yield [ND50] of the biological activity of Rat IFN - γ (0.3 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 Ifng
全名 interferon gamma
背景介绍 This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
生物功能 Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
产品亮点 Related products:
IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Anti-Goat IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
预测分子量 19 kDa
翻译后修饰 Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

检测图片 (3) Click the Picture to Zoom In

  • ARG56608 anti-IFN gamma antibody WB image

    Western blot: 250 - 0.24 ng of Rat IFN-γ stained with ARG56608 anti-IFN gamma antibody, under reducing conditions.

  • ARG56608 anti-IFN gamma antibody WB image

    Western blot: 250 - 0.24 ng of Rat IFN-γ stained with ARG56608 anti-IFN gamma antibody, under non-reducing conditions.

  • ARG56608 anti-IFN gamma antibody standard curve image

    Sandwich ELISA: ARG56608 anti-IFN gamma antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56723 anti-IFN-gamma antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.