ARG56606

anti-IFN gamma antibody

anti-IFN gamma antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes IFN gamma
反应物种 Hu
应用 ELISA, IHC-P, Neut, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 IFN gamma
抗原物种 Human
抗原 E.coli derived Recombinant Human IFN-γ.
(MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ)
偶联标记 Un-conjugated
別名 IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

应用说明

应用建议
应用 推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml as a capture antibody
IHC-P0.5 µg/ml - 0.25 µg/ml
Neut0.13 - 0.20 µg/ml (To yield [ND50] of the biological activity of Human IFN - γ (10 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3458 Human IFNG

Swiss-port # P01579 Human Interferon gamma

基因名称 IFNG
全名 interferon, gamma
背景介绍 This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
生物功能 Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
产品亮点 Related products:
IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
预测分子量 19 kDa
翻译后修饰 Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

检测图片 (4) Click the Picture to Zoom In

  • ARG56606 anti-IFN gamma antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56606 anti-IFN gamma antibody concentration is 0.5 - 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56606 anti-IFN gamma antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human cervical squamous cell carcinoma. The recommended ARG56606 anti-IFN gamma antibody concentration is 0.5 - 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56606 anti-IFN gamma antibody WB image

    Western blot: 250 - 0.24 ng of Human IFN-γ stained with ARG56606 anti-IFN gamma antibody, under non-reducing conditions.

  • ARG56606 anti-IFN gamma antibody standard curve image

    Sandwich ELISA: ARG56606 anti-IFN gamma antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with anti-IFN-gamma antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.