ARG66715
anti-IFIT1 antibody
anti-IFIT1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IFIT1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IFIT1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide around the N-terminal region of Human IFIT1. |
| 偶联标记 | Un-conjugated |
| 別名 | IFI-56; C56; IFIT-1; ISG56; Interferon-induced 56 kDa protein; IFNAI1; IFI-56K; Interferon-induced protein with tetratricopeptide repeats 1; G10P1; P56; IFI56; RNM561 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 实际分子量 | ~ 58 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 30% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P09914 Human Interferon-induced protein with tetratricopeptide repeats 1 Swiss-port # Q64282 Mouse Interferon-induced protein with tetratricopeptide repeats 1 |
|---|---|
| 基因名称 | IFIT1 |
| 全名 | interferon-induced protein with tetratricopeptide repeats 1 |
| 背景介绍 | This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2012] |
| 生物功能 | Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | Phosphorylated. ISGylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
-
ARG66715 anti-IFIT1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue section. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was stained with ARG66715 anti-IFIT1 antibody at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
ARG66715 anti-IFIT1 antibody WB image
Western blot: H1792, LO2, AML12 and PC12 whole cell lysates stained with ARG66715 anti-IFIT1 antibody.
