ARG59752
anti-IDH3B antibody
anti-IDH3B antibody for Western blot and Mouse
概述
| 产品描述 | Goat Polyclonal antibody recognizes IDH3B |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Hu, Rat, Cow, Dog, Pig |
| 应用 | WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IDH3B |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide coresponding to aa. 369-383 of Human IDH3B. (NP_008830.2; NP_777281.1. C-TTDFIKSVIGHLQTK) |
| 偶联标记 | Un-conjugated |
| 別名 | RP46; Isocitric dehydrogenase subunit beta; NAD; Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial; H-IDHB; EC 1.1.1.41 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||
| 实际分子量 | ~ 40 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IDH3B |
| 全名 | isocitrate dehydrogenase 3 (NAD+) beta |
| 背景介绍 | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| 细胞定位 | Mitochondrion. [UniProt] |
| 预测分子量 | 42 kDa |
检测图片 (1) Click the Picture to Zoom In
-
ARG59752 anti-IDH3B antibody WB image
Western blot: 35 µg of Mouse fetal kidney lysate (in RIPA buffer) stained with ARG59752 anti-IDH3B antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
