ARG65798
anti-IDH2 antibody
anti-IDH2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig
概述
| 产品描述 | Goat Polyclonal antibody recognizes IDH2 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Pig |
| 预测物种 | Cow, Dog |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IDH2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the internal region of Human IDH2. (CIHGLSNVKLNE) |
| 偶联标记 | Un-conjugated |
| 別名 | D2HGA2; IDH; Isocitrate dehydrogenase [NADP], mitochondrial; IDPM; EC 1.1.1.42; mNADP-IDH; ICD-M; IDP; IDHM; NADP; Oxalosuccinate decarboxylase |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IDH2 |
| 全名 | isocitrate dehydrogenase 2 (NADP+), mitochondrial |
| 背景介绍 | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| 生物功能 | Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. [UniProt] |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. |
检测图片 (3) Click the Picture to Zoom In
-
ARG65798 anti-IDH2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65798 anti-IDH2 antibody at 3.75 µg/ml dilution followed by AP-staining.
-
ARG65798 anti-IDH2 antibody WB image
Western blot: 35 µg of Human Heart lysate stained with ARG65798 anti-IDH2 antibody at 0.1 µg/ml dilution (1 hour incubation).
-
ARG65798 anti-IDH2 antibody WB image
Western blot: 35 µg of A) Mouse, B) Rat, and C) Pig heart lysates stained with ARG65798 anti-IDH2 antibody at 0.1 µg/ml dilution (1 hour incubation).
