anti-IDH1 antibody (R132H) [SQab22257]

anti-IDH1 antibody (R132H) [SQab22257] for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Metabolism antibody; Signaling Transduction antibody


产品描述 Recombinant Rabbit Monoclonal antibody [SQab22257] recognizes IDH1 (R132H)
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab22257
同位型 IgG
靶点名称 IDH1
抗原物种 Human
抗原 Synthetic peptide around the region of Human IDH1 R132H.
偶联标记 Un-conjugated
Protein Full name Isocitrate dehydrogenase [NADP] cytoplasmic
別名 IDPC; EC; Cytosolic NADP-isocitrate dehydrogenase; IDP; HEL-S-26; HEL-216; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; PICD; IDCD; NADP; Oxalosuccinate decarboxylase


应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Human glioma (IDH1 R132H) tissue


形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05%BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 3417 Human IDH1

Swiss-port # O75874 Human Isocitrate dehydrogenase [NADP] cytoplasmic

基因名称 IDH1
全名 isocitrate dehydrogenase 1 (NADP+), soluble
背景介绍 Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
产品亮点 Related products:
Isocitrate Dehydrogenase antibodies; Isocitrate Dehydrogenase ELISA Kits; Anti-Rabbit IgG secondary antibodies;
Related news:
TCA intermediate fumarate promotes mitobiogenesis
研究领域 Cancer antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 47 kDa
翻译后修饰 Acetylation at Lys-374 dramatically reduces catalytic activity.

检测图片 (1) Click the Picture to Zoom In

  • ARG66929 anti-IDH1 antibody (R132H) [SQab22257] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human glioma (IDH1 R132H) tissue stained with ARG66929 anti-IDH1 antibody (R132H) [SQab22257]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).