ARG64293
anti-IDE / Insulysin antibody
anti-IDE / Insulysin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes IDE / Insulysin |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_004960.1 and NP_001159418.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IDE / Insulysin |
| 抗原物种 | Human |
| 抗原 | C-QQYNFDRDNTE |
| 偶联标记 | Un-conjugated |
| 別名 | Insulysin; Abeta-degrading protease; Insulinase; EC 3.4.24.56; Insulin protease; INSULYSIN; Insulin-degrading enzyme |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IDE |
| 全名 | insulin degrading enzyme |
| 背景介绍 | This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009] |
| 生物功能 | Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 118 kDa |
| 翻译后修饰 | The N-terminus is blocked. |
检测图片 (4) Click the Picture to Zoom In
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ARG64293 anti-IDE / Insulysin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human Lung. (Steamed antigen retrieval with Citrate buffer pH 6) stained with ARG64293 anti-IDE / Insulysin antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG64293 anti-IDE / Insulysin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64293 anti-IDE / Insulysin antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG64293 anti-IDE / Insulysin antibody WB image
Western blot: K562 cell lysate (35 µg protein in RIPA buffer) stained with ARG64293 anti-IDE / Insulysin antibody at 0.1 µg/ml dilution.
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ARG64293 anti-IDE / Insulysin antibody WB image
Western blot: 1). Mock transfection; 2) Insulysin expressing plasmid transfected HEK293 cell lysate stained with ARG64293 anti-IDE / Insulysin antibody.
