ARG57122
anti-ICT1 antibody [1E9]
anti-ICT1 antibody [1E9] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1E9] recognizes ICT1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1E9 |
| 同位型 | IgG1, kappa |
| 靶点名称 | ICT1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 30-206 of Human ICT1 |
| 偶联标记 | Un-conjugated |
| 別名 | 39S ribosomal protein L58, mitochondrial; DS-1; MRP-L58; DS1; Peptidyl-tRNA hydrolase ICT1, mitochondrial; EC 3.1.1.29; Digestion substraction 1; Immature colon carcinoma transcript 1 protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14197 Human Peptidyl-tRNA hydrolase ICT1, mitochondrial |
|---|---|
| 基因名称 | ICT1 |
| 全名 | immature colon carcinoma transcript 1 |
| 背景介绍 | The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014] |
| 生物功能 | Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. [UniProt] |
| 预测分子量 | 24 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57122 anti-ICT1 antibody [1E9] FACS image
Flow Cytometry: A549 cell line stained with ARG57122 anti-ICT1 antibody [1E9] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG57122 anti-ICT1 antibody [1E9] WB image
Western blot: 40 µg of A549 and HeLa cell lysates stained with ARG57122 anti-ICT1 antibody [1E9] at 1:1000.
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ARG57122 anti-ICT1 antibody [1E9] WB image
Western blot: 5 µg of 1) 293T, and 2) ICT Transfected 293T cell lysate stained with ARG57122 anti-ICT1 antibody [1E9] at 1:1000.
