ARG54402
anti-ICAD antibody
anti-ICAD antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ICAD |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | This antibody recognizes non-cleaved (45kDa) and cleaved DFF. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ICAD |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to aa 2-21 at the N-terminus of human DFF45 (accession no. NP_004392). |
| 偶联标记 | Un-conjugated |
| 別名 | DFF-45; DNA fragmentation factor 45 kDa subunit; Inhibitor of CAD; ICAD; DFF1; DNA fragmentation factor subunit alpha |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HeLa, K562, Jurkat and Raji |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunoaffinity chroma-tography |
| 缓冲液 | PBS (pH 7.4) and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O00273 Human DNA fragmentation factor subunit alpha |
|---|---|
| 基因名称 | DFFA |
| 全名 | DNA fragmentation factor, 45kDa, alpha polypeptide |
| 背景介绍 | A human DNA fragmentation factor (DFF) which is cleaved by caspase-3 during apoptosis was identified recently. The mouse homologue of human DFF was identified as a DNase inhibitor and was designated ICAD. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in nuclei. Cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is a hallmark of apoptotic cell death. |
| 生物功能 | Inhibitor of the caspase-activated DNase (DFF40). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody |
| 预测分子量 | 37 kDa |
| 翻译后修饰 | Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. |
检测图片 (1) Click the Picture to Zoom In
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ARG54402 anti-ICAD antibody WB image
Western blot: H:HeLa; K:K562; J:Jurkat; R:Raji stained with ARG54402 anti-ICAD antibody at 2 μg/ml dilution.
