ARG59815
anti-IARS / Isoleucyl tRNA synthetase antibody
anti-IARS / Isoleucyl tRNA synthetase antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IARS / Isoleucyl tRNA synthetase |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IARS / Isoleucyl tRNA synthetase |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1158-1262 of Human IARS (NP_002152.2). |
| 偶联标记 | Un-conjugated |
| 別名 | ILERS; IARS1; EC 6.1.1.5; IleRS; IRS; ILRS; Isoleucyl-tRNA synthetase; Isoleucine--tRNA ligase, cytoplasmic; PRO0785 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Jurkat, HL-60 and HepG2 | ||||
| 实际分子量 | 160 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P41252 Human Isoleucine--tRNA ligase, cytoplasmic |
|---|---|
| 基因名称 | IARS |
| 全名 | isoleucyl-tRNA synthetase |
| 背景介绍 | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012] |
| 细胞定位 | Cytoplasm. Cytoplasm, cytosol. [UniProt] |
| 预测分子量 | 144 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG59815 anti-IARS / Isoleucyl tRNA synthetase antibody WB image
Western blot: 25 µg of Jurkat, HL-60 and HepG2 cell lysates stained with ARG59815 anti-IARS / Isoleucyl tRNA synthetase antibody at 1:1000 dilution.
