ARG53947
anti-Hsp 90 beta antibody [MBH90B]
anti-Hsp 90 beta antibody [MBH90B] for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Bovine
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MBH90B] recognizes Hsp 90 beta |
|---|---|
| 反应物种 | Hu, Ms, Bov |
| 应用 | ICC/IF, IP, WB |
| 特异性 | The clone MBH90B recognizes the EEVHHG epitope within the N-terminal part of Hsp90 beta an ubiquitously expressed protein with calculated Mw of 83.3 kDa, however, migrating as a 90 kDa band under reducing SDS-PAGE conditions. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MBH90B |
| 同位型 | IgG1 |
| 靶点名称 | Hsp 90 beta |
| 抗原物种 | Human |
| 抗原 | Peptide corresponding to the EEVHHGEEEVEC sequence within N-terminal part of human Hsp90. |
| 偶联标记 | Un-conjugated |
| 別名 | HSPC2; D6S182; Heat shock 84 kDa; HSP90B; HSP84; HSP 84; Heat shock protein HSP 90-beta; HSP 90; HSPCB |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSP90AB1 |
| 全名 | heat shock protein 90kDa alpha (cytosolic), class B member 1 |
| 背景介绍 | Hsp90 beta (heat shock protein 90 beta) is a constitutively expressed isoform of Hsp90, one of the most abundant chaperones in the cytosol of eukaryotic cells. Hsp90 interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation. Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis. |
| 生物功能 | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 83 kDa |
| 翻译后修饰 | Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro). ISGylated. S-nitrosylated; negatively regulates the ATPase activity. Phosphorylation at Tyr-301 by SRC is induced by lipopolysaccharide (PubMed:23585225). Phosphorylation at Ser-226 and Ser-255 inhibits AHR interaction (PubMed:15581363). Methylated by SMYD2; facilitates dimerization and chaperone complex formation; promotes cancer cell proliferation. Cleaved following oxidative stress resulting in HSP90AB1 protein radicals formation; disrupts the chaperoning function and the degradation of its client proteins. |
检测图片 (1) Click the Picture to Zoom In
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ARG53947 anti-Hsp 90 beta antibody [MBH90B] WB image
Western blot: Various cell lines and recombinant Hsp90 alpha and Hsp90 beta protein stained with ARG53947 anti-Hsp 90 beta antibody [MBH90B].
克隆号文献
LPS induces pp60c-src-mediated tyrosine phosphorylation of Hsp90 in lung vascular endothelial cells and mouse lung.
Impaired infectivity of ritonavir-resistant HIV is rescued by heat shock protein 90AB1.
WB / Human
