ARG54021
anti-Hsp 70 antibody (C-term)
anti-Hsp 70 antibody (C-term) for Immunoprecipitation,Western blot and Human
Cancer antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes Hsp 70 |
|---|---|
| 反应物种 | Hu |
| 应用 | IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG2b |
| 靶点名称 | Hsp 70 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human Hsp70 (C-terminus) protein fragments expressed in E. coli. |
| 偶联标记 | Un-conjugated |
| 別名 | Heat shock 70 kDa protein 1A; HSPA1; HSP70I; Heat shock 70 kDa protein 1; HSP70-1A; HEL-S-103; HSP70.1; HSP72; HSP70-1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide, 0.1mg/ml BSA and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 0.1mg/ml BSA, 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSPA1A |
| 全名 | heat shock 70kDa protein 1A |
| 背景介绍 | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008] |
| 生物功能 | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. [UniProt] |
| 研究领域 | Cancer antibody; Signaling Transduction antibody |
| 预测分子量 | 70 kDa |
| 翻译后修饰 | In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG54021 anti-Hsp 70 antibody (C-term) WB image
Western blot: HeLa cell lysate stained with ARG54021 anti-Hsp 70 antibody (C-term) at 1:1000 dilution.
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ARG54021 anti-Hsp 70 antibody (C-term) IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54021 anti-Hsp 70 antibody (C-term).
