ARG10757

anti-Hsp 60 antibody

anti-Hsp 60 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human

Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
publication_link 参考文献3

概述

产品描述 Chicken Polyclonal antibody recognizes Hsp 60
反应物种 Hu
应用 ICC/IF, IHC-Fr, WB
宿主 Chicken
克隆 Polyclonal
同位型 IgY
靶点名称 Hsp 60
抗原物种 Human
抗原 Recombinant full length purified HSP60 from E. coli.
偶联标记 Un-conjugated
別名 Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:2000 - 1:5000
IHC-Fr1:2000 - 1:5000
WB1:10000 - 1:20000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
缓冲液 PBS and 0.02% Sodium azide.
抗菌剂 0.02% Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3329 Human HSPD1

Swiss-port # P10809 Human 60 kDa heat shock protein, mitochondrial

基因名称 HSPD1
全名 heat shock 60kDa protein 1 (chaperonin)
背景介绍 This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
生物功能 Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
产品亮点 Related products:
Hsp60 antibodies; Hsp60 Duos / Panels; Anti-Chicken IgY secondary antibodies;
Related poster download:
Organelle Markers & Loading Control
研究领域 Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody
预测分子量 61 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG10757 anti-Hsp 60 antibody ICC/IF image

    Immunocytochemistry: HeLa cells were stained with ARG10757 anti-Hsp 60 antibody (green). Blue is DAPI staining of DNA. ARG10757 reveals strong staining in mitochondria.

  • ARG10757 anti-Hsp 60 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG10757 anti-Hsp 60 antibody (green) at 1:5000 dilution and costained with Mouse mAb to actin (red) at 1:500 dilution. DAPI (blue) for nuclear staining.

    The Hsp 60 antibody produces strong and specific staining of mitochondria, while the actin antibody labels the submembranous cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

  • ARG10757 anti-Hsp 60 antibody WB image

    Western blot: 1) SHSY-5Y, and 2) HeLa cell lysates stained with ARG10757 anti-Hsp 60 antibody at 1: 20,000 dilution.

文献引用

Development and characterization of phospho-ubiquitin antibodies to monitor PINK1-PRKN signaling in cells and tissue

ICC/IF / Human

Jens O Watzlawik et al.
bioRxiv,  (2024)

publication_link

 

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Alpha-synuclein-associated changes in PINK1-PRKN-mediated mitophagy are disease context dependent

ICC/IF / Human

Xu Hou et al.
Brain Pathol.,  (2023)

publication_link

 

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Systematic Functional Analysis of PINK1 and PRKN Coding Variants

ICC/IF / Human

Benjamin J Broadway et al.
Cells,  (2022)

publication_link

 

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