ARG56900
anti-Hsp 40 antibody
anti-Hsp 40 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes Hsp 40 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Hsp 40 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-340 of Human Hsp 40 (NP_006136.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Hsp40; HSP40; DnaJ protein homolog 1; hDj-1; HSPF1; Human DnaJ protein 1; Hdj1; RSPH16B; Heat shock 40 kDa protein 1; DnaJ homolog subfamily B member 1; Heat shock protein 40; Sis1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Rat testis, Mouse thymus and BxPC-3 | ||||||
| 实际分子量 | 40 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | DNAJB1 |
| 全名 | DnaJ (Hsp40) homolog, subfamily B, member 1 |
| 背景介绍 | This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| 生物功能 | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. [UniProt] |
| 预测分子量 | 38 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG56900 anti-Hsp 40 antibody WB image
Western blot: 25 µg of Rat testis, Mouse thymus and BxPC-3 cell lysates stained with ARG56900 anti-Hsp 40 antibody at 1:1000 dilution through one-step method.
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ARG56900 anti-Hsp 40 antibody ICC/IF image
Immunofluorescence: C6 cells stained with ARG56900 anti-Hsp 40 antibody at 1:100 dilution.
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ARG56900 anti-Hsp 40 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG56900 anti-Hsp 40 antibody at 1:100 dilution.
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ARG56900 anti-Hsp 40 antibody WB image
Western blot: Extracts from normal (Control) and Hsp 40 knockout (KO) 293T cells, using ARG56900 anti-Hsp 40 antibody at 1:1000 dilution.
