ARG22196

anti-Hsp 40 antibody

anti-Hsp 40 antibody for ICC/IF,Immunoprecipitation,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes Hsp 40
反应物种 Hu
应用 ICC/IF, IP, WB
特异性 Detects ~40kDa.
宿主 Rabbit
克隆 Polyclonal
靶点名称 Hsp 40
抗原物种 Human
抗原 Recombinant purified Human HSP40
偶联标记 Un-conjugated
別名 Hsp40; HSP40; DnaJ protein homolog 1; hDj-1; HSPF1; Human DnaJ protein 1; Hdj1; RSPH16B; Heat shock 40 kDa protein 1; DnaJ homolog subfamily B member 1; Heat shock protein 40; Sis1

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100
IPAssay-dependent
WB1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Rabbit antiserum.
缓冲液 Rabbit Antiserum
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 3337 Human DNAJB1

Swiss-port # P25685 Human DnaJ homolog subfamily B member 1

基因名称 DNAJB1
全名 DnaJ (Hsp40) homolog, subfamily B, member 1
背景介绍 This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
生物功能 Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. [UniProt]
细胞定位 Cytoplasm, Nucleus
预测分子量 38 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG22196 anti-Hsp 40 antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22196 anti-Hsp 40 antibody (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22196 anti-Hsp 40 antibody WB image

    Western blot: Human HeLa cell lysates stained with ARG22196 anti-Hsp 40 antibody at 1:1000 dilution.

  • ARG22196 anti-Hsp 40 antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22196 anti-Hsp 40 antibody (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.