ARG55955
anti-Hsp 27 antibody [G3.1]
anti-Hsp 27 antibody [G3.1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [G3.1] recognizes Hsp 27 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | G3.1 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Hsp 27 |
| 抗原物种 | Human |
| 抗原 | Partially purified Human Hsp27 (earlier called 24K) from breast cancer MCF-7 cells. |
| 偶联标记 | Un-conjugated |
| 別名 | HSP 27; Heat shock 27 kDa protein; HMN2B; HS.76067; SRP27; HEL-S-102; HspB1; CMT2F; 28 kDa heat shock protein; HSP27; Heat shock protein beta-1; Hsp25; Estrogen-regulated 24 kDa protein; Stress-responsive protein 27; HSP28 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HSPB1 |
| 全名 | heat shock 27kDa protein 1 |
| 背景介绍 | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| 生物功能 | Involved in stress resistance and actin organization. [UniProt] |
| 细胞定位 | Predominantly cytoplasmic with some nuclear |
| 预测分子量 | 23 kDa |
| 翻译后修饰 | Phosphorylated upon exposure to protein kinase C activators and heat shock (PubMed:8325890). Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement (PubMed:1332886, PubMed:8093612, PubMed:19166925). |
检测图片 (3) Click the Picture to Zoom In
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ARG55955 anti-Hsp 27 antibody [G3.1] IHC-P image
Immunohistochemistry: Human breast carcinoma (10X) stained with ARG55955 anti-Hsp 27 antibody [G3.1].
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ARG55955 anti-Hsp 27 antibody [G3.1] IHC-P image
Immunohistochemistry: breast carcinoma (20X) stained with ARG55955 anti-Hsp 27 antibody [G3.1].
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ARG55955 anti-Hsp 27 antibody [G3.1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human prostate carcinoma stained with ARG55955 anti-Hsp 27 antibody [G3.1].
